Similarly as we reported for RKO colon cancer cells [14], depletion of TSG101 or VPS28 in HEK293 cells led to a strong intracellular accumulation of ubiquitin (Fig. 2E), likely due to the impairment of multiple ESCRT-I-mediated degradation processes [14]. The gene discussed is VPS28; the disease is malignant colon neoplasm.