When focusing on only one sample, such as the HER2‐positive breast cancer sample PM0337, scPharm completed a pharmacological subpopulation analysis for a single drug within 1 min across a range of 500 to 2000 cells, which was significantly less time‐consuming than that of all other tools, except SeuratCCA (Figure 5G). The gene discussed is ERBB2; the disease is breast cancer.