For example, Threonine (Thr) Thr 181, Thr 217, and Thr 231 hyperphosphorylation has been reported in the early preclinical stages of AD,41 whereas AT8 Serine (Ser) (Ser202/Thr205) and PHF1 (Ser396/Ser404) hyperphosphorylation are reported in later stages of AD.42, 43. This evidence concerns the gene PHF1 and Alzheimer disease.