We identified 16 distinct clusters; based on literature (31, 32) and marker expression we were able to assign these clusters into epithelial (MB49 tumor cluster, Krt8+, Krt18+), uroplakin-enriched cluster representing the normal urothelium of the bladder (Krt8+, Krt18+, Krt19+, Krt5+, Upk1a+, Upk1b+, Upk2+ and Upk3a+), endothelial cluster (Vcam+), T-cell cluster (Cd3d+, Cd3g+, Cd3e+), macrophages (Adgre1+, Csf1r+, Mafb+, Cd14+, Fcgr+), and neutrophils (Csf3r+, S100a9+, S100a9+, Cxcl3+). This evidence concerns the gene UPK2 and neoplasm.