LGR5 and neoplasm: We next assessed the effect of excess asparagine or asparaginase-deficient STm on tumour organoid expression of the stem cell marker Lgr5, as we have previously reported that STmΔaroA causes a reduction of multiple indications of tumour stemness, including expression of Lgr5. There was a trend for a reduction in Lgr5 expression by STmΔaroA, which was abrogated by asparagine or the asparaginase mutant (Fig. 8E).