The use of sex-specific cut-offs, as suggested in a study from the US evaluating the predictive value of 3-hr change of hs-cTnT with respect to acute myocardial infarction and major adverse cardiac events16, lead in our sample to a reduction in the number of participants with undetectable levels at baseline but levels higher than the respective sex-specific cut-offs at follow-up. This evidence concerns the gene TNNT2 and acute myocardial infarction.