To elucidate the molecular mechanisms of NOTCH3 promoting the malignant progression of BLCA, RNA sequencing was performed on T24 cells with NOTCH3 knockdown, revealing 422 differentially expressed genes positively correlated with NOTCH3 (P < 0.05, |fold change| >1) (Fig. 3A, B). The gene discussed is NOTCH3; the disease is bladder transitional cell carcinoma.