Based on self‐developed multi‐real‐time digital PCR (MRT‐dPCR) and algorithms, d‐SCOUT allows for the unified quantification of Asialoglycoprotein Receptor (ASGPR), Glypican‐3 (GPC‐3), and Epithelial Cell Adhesion Molecule (EpCAM) proteins, as well as Programmed Death Ligand 1 (PD‐L1), GPC‐3, and EpCAM mRNA in HCC CTCs, with good sensitivity (LOD of 3.2 CTCs per mL of blood) and reproducibility (mean %CV = 1.80–6.05%). This evidence concerns the gene CD274 and hepatocellular carcinoma.