As depicted in Figure4a, the distribution of endpoint wells positive for HCC CTC proteins (EpCAM, GPC‐3, and ASGPR) and mRNAs (EpCAM, GPC‐3, and PD‐L1) in the FAM, VIC, and CY5 channels was clearly demonstrated. The gene discussed is ASGR1; the disease is hepatocellular carcinoma.