As shown in Fig 4C and 4D, the number and percentages of IFN-γ+ CD4+ T cells, IFN-γ+ γδ+ T cells, and particularly IL-17A+ CD4+ T cells and IL-17A+ γδ+ T cells in lung of EPS301-adjuvanted mice was markedly higher than that in other groups on day 112 post vaccination in response to infection. This evidence concerns the gene CD4 and infection.