It has also been confirmed that the upregulation of PCNA expression leads to a poor prognosis in patients with osteosarcoma.53,54 In addition, studies have reported that increased ASF1B expression can promote the malignant proliferation of a variety of tumor cells, such as renal cell carcinoma, breast, prostate, and cervical cancer.55–57 There is a good chance that the synergy between DSN1 and the co-expressed genes contributes to LGG development. This evidence concerns the gene PCNA and renal cell carcinoma.