Recently, we identified a gain-of-function mutation in TRPM8 (R30Q) in a patient with trigeminal neuralgia and demonstrated that it confers multiple gain-of-function attributes, i.e., enhanced activation, increased basal [Ca2+]i, and enhanced menthol response, on the channel.13 In this study, we assessed D665N and V915M, 2 mutations in the N-terminus and pore loop of TRPM8, respectively, that were found in patients with persistent ocular pain after corneal refractive surgery. This evidence concerns the gene TRPM8 and trigeminal nerve disorder.