CASP1 and Anxiety: In our study, the protein expression of NLRP3, Asc, and Caspase-1 were activated in the mPFC and Hip regions of CRS mice, whereas JSO administration suppressed NLRP3 inflammasome activation, indicating that the anti-anxiety and anti-depressant outcome of treatment with JSO may be partly mediated via inhibition of NLRP3 inflammasome activation.