TNF and Familial prostate cancer: Another study conducted by Lopes and Callera (2012) suggested that the levels of IL-5, IL-4, IL-2, TNF-α, and MIP-1α were not significantly affected by irradiation in three-dimensional conformal radiotherapy of prostate cancer, while the levels of IL-6 seemed to reach their highest point after a duration of 15 days of radiotherapy.