We and others have previously demonstrated that iPSCs derived from human AML can be re-differentiated into a transplantable human leukemia that re-acquires both phenotypic and epigenetic features of the original patient sample.28,29 To assess the role of DNMT3AR882H in leukemic maintenance, we established an AML-derived iPSC line (iSU444) that carries the DNMT3AR882H mutation along with FLT3-ITD, but otherwise has a normal karyotype (Figure S4A). This evidence concerns the gene FLT3 and acute myeloid leukemia.