Our experiments showed that compared with the MYOF+TAC group, NLRP3 overexpression can reverse the protective effects of MYOF, increase the proportion of apoptotic cells (Fig. 4F), the HW/BW ratio (Fig. 4G), cardiomyocyte cross-sectional area (Figs. 4H and 4I), myocardial fibrosis area (Figs. 4J and 4K), and weaken cardiac function (Figs. 4L and 4M). The gene discussed is MYOF; the disease is Myocardial fibrosis.