In addition, we performed enrichment analysis (including Gene Ontology [GO] and Kyoto Encyclopedia of Genes and Genomes [KEGG]) on genes with differential expression and analyzed the expression of nine checkpoints including vascular endothelial growth factor A (VEGFA), cluster of differentiation 27 (CD27), interleukin‐2 receptor alpha (IL2RA), etc. discovering a significant association between FOXK2 expression and immune cell infiltration, activation of anti‐cancer immunity, and the survival rate of patients undergoing immunotherapy. The gene discussed is CD27; the disease is cancer.