These of our preliminary data encouraged us to assess the effect of single depletion of PML, DAXX, MAD2, and TSG101 on cellular events, including mitochondrial function, colocalization between mitochondria and LC3B (mitophagy-like status), PML NB formation, and colocalization between mitochondria and PML NBs which implies PML-MAMs (Mitochondria-associated endoplasmic reticulum membranes) interaction that modulates autophagy, tumor immune microenvironment, and cancer development [35, 36] (Fig. S5C–S5P; Supplemental Result 1). The gene discussed is TSG101; the disease is cancer.