The results of our TUNEL assay are consistent with the results of our colony outgrowth assay and zebrafish xenograft implanted with human cells, including HSkMC, HeLa, and human rhabdomyosarcoma cell line Rh30, which shows a higher protein level of MAD2 (Fig. 1H–J and S3A–S3G; Table S5 for simplified sample indications). This evidence concerns the gene MAD2L1 and rhabdomyosarcoma.