Further rescue experiments confirmed that Zn2+ could alleviate high-fat–induced lipid metabolic disorders, inhibit the activation of AKT/PI3K pathway and the expression of inflammatory factors in vitro and in vivo, but these effects were reversed by Mt1b silencing (Fig. 7A–L; Supplementary Fig. S6A–D). The gene discussed is AKT1; the disease is Disorder of lipid metabolism.