In an earlier study, we have compared tritosomes with a more crudely enriched 20,000 × g fraction, showing that changes in protein amounts between tritosomes from wildtype and Cln6-deficient mice, which display a lysosomal storage disorder, could be reproduced in lysosomal fractions devoid of Triton WR133945. Here, CLN6 is linked to lysosomal storage disease.