To investigate the effect of Myo9 motor inhibition in cancer cells, we chose different epithelial human and mouse-derived melanoma and carcinoma cell lines (A549, MLE-12, B16-F1, Caco-2, and Calu-3), which express both Myo9a and Myo9b encoding transcripts at comparable levels as healthy lung tissue or higher (Supplementary Fig. 2A, B). This evidence concerns the gene MYO9B and carcinoma.