AKT1 and breast carcinoma: In the SVP dropdown list under the Pathway option, multiple signaling pathways with highly variable spatial activity on the slice were identified, including the ‘MAPK signaling pathway’, ‘PI3K-Akt signaling pathway’, ‘mTOR signaling pathway’, ‘VEGF signaling pathway’, etc. These signaling pathways have been confirmed to be oncogenic pathways and are closely associated with breast cancer (38–40).