Unsupervised hierarchical clustering of TCGA primary tumor and matched normal samples (n=1201) based on expression of LEP-derived age-dependent DE and DV genes (k=589 genes) in the TCGA cohort identified four main sample clusters (Figure 7A): (i) cluster 1 represented predominantly by PAM50 LumA and Her2 breast cancer subtypes; (ii) cluster 2 by PAM50 LumB and LumA subtypes; (iii) cluster 3 by PAM50 Basal subtype; and (iv) cluster 4 by matched normal samples. This evidence concerns the gene TMEM43 and breast cancer.