To determine whether knockdown of Cbx3 in murine glioma cell lines regulates phagocytosis, we collected the brains from mice bearing luciferase-expressing CT2A tumors and assayed phagocytosis with flow cytometry, using anti-luciferase antibody to detect tumor cells and antibodies against microglial markers CD11b and Tmem119, showing that knockdown of murine Cbx3 in CT2A cells increased phagocytosis (Supplemental Figure 7, G–J). This evidence concerns the gene ITGAM and central nervous system cancer.