CD68 and neoplasm: Archival formalin-fixed paraffin-embedded (FFPE) tumour tissue of this node was interrogated for CD3, CD4, CD8, CD103, granzyme B, CD68, PD-1, PD-L1, MHC class II and pan-cytokeratin (panCK) expression using multispectral immunohistochemistry, as previously described (10).