Comparison of transcriptomes from cocultured CD4+ T cells and SK-Mel-147 ctrl vs. their corresponding monocultures revealed a total of 9,453 and 4,521 DEGs (P<0.05, Figure 3D), indicating a strong reciprocal induction of transcriptional changes in E2F1-abundant melanoma and immune cells through a cytokine-mediated crosstalk. Here, CD4 is linked to melanoma.