ENG and myelodysplastic syndrome: developed a protocol using CD36, CD71, CD105, CD117, CD13 and CD45, combined with a no-lysis protocol, which provided a more reliable evaluation of erythroid dysplasia and led to the integration of artificial intelligence (AI) in the form of Flow-Self Organizing Maps algorithm (FlowSOM) unsupervised analysis, to define distinct clusters within the erythroid compartment in normal BM and subsequently in MDS (94, 106, 107).