developed a protocol using CD36, CD71, CD105, CD117, CD13 and CD45, combined with a no-lysis protocol, which provided a more reliable evaluation of erythroid dysplasia and led to the integration of artificial intelligence (AI) in the form of Flow-Self Organizing Maps algorithm (FlowSOM) unsupervised analysis, to define distinct clusters within the erythroid compartment in normal BM and subsequently in MDS (94, 106, 107). This evidence concerns the gene ANPEP and myelodysplastic syndrome.