Pretreatment with NOX4 siRNA, NAC (an ROS scavenger), and VAS2870 (an NOX4 inhibitor) significantly reduced the expression levels of NLRP3, cleaved caspase-1, ASC, cleaved IL-1β, and GSDMD-N in the CB-treated HCC cells. The gene discussed is IL1B; the disease is hepatocellular carcinoma.