Many studies have shown that Notch1 is involved in the tumor growth, metastasis, drug resistance and recurrence of a variety of tumors, especially its close relationship with glioma stem cells.[39, 40, 41, 42, 43] In this study, we speculated that Notch1 could be a key molecule in the TMZ resistance of gliomas and subsequently constructed Notch1 knockout GL261 cell lines. Here, NOTCH1 is linked to central nervous system cancer.