In comparison with NSCLC cells overexpressing empty vector as a negative control (NC), MDM2 readily induced a stable resistant phenotype to Osimertinib in PC-9 and HCC827 cells, as demonstrated by MTT cell viability assay and colony formation assay (Fig. 2A–C, Fig. S2A-S2C). This evidence concerns the gene MDM2 and non-small cell lung carcinoma.