OPTN and glaucoma: As astrocytes serve in numerous important roles, including critical contributions to the neurovascular unit, and previous studies have demonstrated that vascular dysfunction is also a characteristic of glaucoma [8, 49, 50], we sought to explore whether hPSC-derived RGCs and astrocytes with the OPTN(E50K) mutation could similarly modulate barrier properties in an established Transwell® model of the neurovascular unit.