Knockdown of PU.1 by a tetracycline-inducible shRNA in THP-1 cells (Schuetzmann et al, 2018) led to enhanced expression of the aforementioned genes, confirming that these genes were downstream by PU.1 in human AML cells (Fig. 4C; Appendix Fig. S4A). Here, SPI1 is linked to acute myeloid leukemia.