Interestingly, the top five upstream regulators that were differentially regulated in the liver of Mll4+/− mice were identified by functional analysis of RNA-seq data, including lipopolysaccharide (LPS), TGFβ1, IFNγ, and TNF, which are known NASH-promoting factors (Fig. 3C). The gene discussed is KMT2D; the disease is metabolic dysfunction-associated steatohepatitis.