By contrast, Hanna et al. (19) reported apparently increased methylation at GNAS-AS2:TSS-DMR in 3 affected members of an AD-PHP1B family who did not have an STX16 deletion, but these individuals differed from those whom we studied as they did not have evidence for global methylation defects and showed normal methylation at GNAS-NESP:TSS-DMR, GNAS-XL:EX1-DMR, and GNAS-AS1:TSS-DMR. This evidence concerns the gene GNAS and Alzheimer disease.