SPOP and malignant colon neoplasm: Based on the levels of RIPK3 and SPOP endogenous expression in colon cancer cell lines, including HT-29, HCT115, DLD-1, HCT116, WiDr, and SW480 (Supplementary Fig. 1A), HT-29 cells were selected for further experiments because they exhibited detectable levels of RIPK3 and SPOP, making them suitable for the present study.