The rotation of the central stalk relative to the surrounding ATP5F1A3ATP5F1B3 subunits leads to hydrolysis of ATP.[14, 15] Numerous studies have demonstrated that ATP synthase can influence prostate cancer development through ATP production.[16, 17] Further RNA sequencing analysis demonstrated that the regulation of ATPase activity was enriched in SFT2D2‐TBX19 overexpression group, but not in the TBX19‐202 overexpression group (Figure 4G,H). This evidence concerns the gene SFT2D2 and Familial prostate cancer.