The analysis of immune cell infiltration showed notable variations in the quantities of different immune cells in normal and sepsis samples, as well as in groups with high and low expression of the hub genes DNMT1, TP53, and TLR8. DNMT1 and TP53 showed a strong positive relationship with T cells CD8 (R = 0.63 for both) and a negative relationship with neutrophils (R = −0.69 and R = −0.62, respectively). The gene discussed is CD8A; the disease is Sepsis.