Wang and team used CRISPR screening of PD1+ HER2+ CAR-T cells and glioblastoma stem cells to find that knocking out Transducin-Like Enhancer of split 4 (TLE4) reduced apoptotic traits, sustaining CAR-T cell function, while ablation of Ikaros family zinc finger protein 2 (IKZF2) improved CAR-T anti-tumor efficacy by regulating NFAT signaling [64]. The gene discussed is IKZF2; the disease is glioblastoma.