In a bilateral melanoma tumour model (using B16-OVA cells), combination of anti-PD-L1 antibody and Mn-MOF/CpG nanoplatform with US irradiation (1 MHz, 1 W/cm2, DC = 50%, for 10 mins (n = 3; five-day intervals)) significantly delayed the growth of both irradiated primary tumours and non-irradiated distant tumours. This evidence concerns the gene CD274 and neoplasm.