TUNEL assay The results revealed that the number of apoptotic tumor cells (brown, dark staining) was significantly greater in the LV-OE-SPINK13 group than in the scramble group, with scores of 3.67 ± 1.70 and 0.07 ± 0.00, respectively, which were significantly different between the two groups (Fig. 5A). The gene discussed is SPINK13; the disease is neoplasm.