Further studies have shown that SPINK13 can significantly inhibit the invasion and migration of hepatoma cells in vitro and the subcutaneous growth of hepatoma cells in nude mouse xenograft tumors by directly inhibiting uPA activity and indirectly inhibiting the activation of MMPs by interacting with uPA in the extracellular space, and the inhibitory effect is significantly dose-dependent [4–7]. Here, PLAU is linked to hepatocellular carcinoma.