We utilized SG deficient U2OS cells (human osteosarcoma epithelial cell line) genetically lacking both G3BP1 and G3BP2 (ΔΔG3BP1/2) (Kedersha et al, 2016), which are essential factors for SG formation (Guillén-Boixet et al, 2020; Kedersha et al, 2016; Yang et al, 2020) (Fig. EV1A). The gene discussed is G3BP2; the disease is osteosarcoma.