Histopathological analysis of the livers also revealed that RIPK3 deficiency and myeloid-specific caspase-8 loss diminished hepatic steatosis, ballooning, and fibrosis in HFD-fed (and in ND-fed) mice (Figs 2J and K and S5D), whereas flow cytometric analysis suggested a trend toward reduced recruitment of inflammatory neutrophils and monocyte/macrophages in Casp8lox/loxRipk3−/− and Casp8LysMcreRipk3−/− HFD-fed mice, compared with controls (Fig S5E–G). This evidence concerns the gene CASP8 and steatosis.