We transduced CD34+ HSPCs from DBA patients BCH-002 (with a mutation in RPL35A) and BCH-008 (with a mutation in RPS19) with HMD-GFP or hG1E-GATA1 and maintained the cells in erythroid differentiation media for 10 days prior to collection for single-cell RNA sequencing (scRNA-seq). The gene discussed is CD34; the disease is Diamond-Blackfan anemia.