To assess whether proteins involved in 5‐methylcytosinylation of RNA mediate EMT in HCC, we transfected DNMT2 and NSUN1‐7 overexpression plasmids, along with their corresponding empty control plasmids, into HepG2 and Hep3B cells, respectively, to establish stable cell lines. The gene discussed is NOP2; the disease is hepatocellular carcinoma.