We performed the RT-qPCR analysis but observed no significant difference in the premature Tap1/2 expression between Map3k1-WT and Map3k1-mut tumor cells in coculture with splenocytes from OT-I mice, indicating that Map3k1 might regulate Tap1/2 expression in a posttranscriptional manner (Figure 5A). This evidence concerns the gene MAP3K1 and neoplasm.