PCYT2 and colorectal carcinoma: To determine whether PCYT2 also has an inhibitory effect on the metastasis of CRC to the lungs, we also established mouse models by injecting PCYT2-overexpression and control (vehicle) luciferase-labeled metastatic HCT116 cells into BALB/c nude mice (n = 7 per group) (Supplemental Figure 4A).