PET-PCR confirmed mixed species infections comprising the mixes Pf and Po, Pf and Po, Pf and Po, and Pf and Pv for four samples from patients reporting travel to African countries (File S1; Table S6: samples 18GNNVxxx0136PfB1230_S51, 18GNNVxxx0137PfB1230, 18WAPAxxA0138PfB1230_S53, and 18CIPAxxP0139PfB1230, respectively). This evidence concerns the gene PRB4 and infection.