PD-1 immunotherapy can achieved desirable response in cancer patients with sensitive gene mutations, including COL3A1, FGFR, breast cancer susceptibility (BRCA), ataxia telangiectasia-mutated gene (ATM), polymerase epsilon (POLE), DNA polymerase delta 1 (POLD1), NOTCH, KMT2C genes and so on. This evidence concerns the gene POLE and cancer.