To better mimic the continuous and dynamic nature of PMN remodeling by tumorous SPP1 during HCC progression and the subsequent metastasis cascade, we overexpressed SPP1 in Hepa1-6 cells (Figure S1N-O) and established a modified Hepa1-6SPP1 or Hepa1-6vector orthotopic spontaneous metastasis model with matched conditioned medium (CM) to accelerate lung remodeling [28]. The gene discussed is SPP1; the disease is hepatocellular carcinoma.