SPP1 and hepatocellular carcinoma: Similarly, SPP1-PMN was observed to enhance early colonization of murine Hepa1-6GFP cells by intravenous injection (i.v) (Fig. 1E) [27], which was concomitant with an increase in experimental metastasis in both i.v. injected Hepa1-6 and H22 cells, a suspended murine HCC cell line from malignant ascites (Fig. 1F).