To further investigate the effect of the DUSP6 M62I mutation on the sensitivity of NSCLC to Osimertinib, we overexpressed wild-type (WT) DUSP6 and M62I mutant DUSP6 in PC9 and H1975 cell lines, and evaluated DUSP6 protein level using Western blot. This evidence concerns the gene DUSP6 and non-small cell lung carcinoma.