In vivo analysis of the effect of the R67 arginine mutant of ME2 on HCC cells using a nude mouse subcutaneous tumor formation assay and statistical analysis of the weight and volume of subcutaneous tumors were also performed, and the results also demonstrated that the R67 arginine mutant of ME2 in vivo could attenuate the growth-promoting effect of the wild-type ME2 on the growth of HCC cells with knockdown of ME2 expression (Fig. 4F–H). Here, ME2 is linked to hepatocellular carcinoma.