ME2 and hepatocellular carcinoma: It has been previously reported that arginine 67 (R67) is a methylated residue [29], so we constructed an arginine mutant at the R67 position by mutating R67 to lysine (ME2-R67K) and compared this mutant to ME2 wild-type (ME2-WT) to analyze the effect of ME2 methylation on hepatocellular carcinoma development.